Our new Distinct+ tissue clearing approach is the one system that delipidates samples without having change in morphology and with minimum effect on structural integrity.
Megatome can be a vibrating microtome built to portion a broad number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables substantial-throughput tissue sectioning with uniform area profile, and minimal tissue damage and information loss.
Antibodies may perhaps choose months to diffuse as a result of only some millimeters of tissue, which has a steep labeling gradient from area to Main.
Megatome is suitable for precision: the blade vibrates at an increased frequency and bigger amplitude array than other microtomes, and contains a exclusive deflection Management system.
SE employs a rotational electrical area to disperse extremely electromobile molecules (such as antibodies or surfactant micelles) throughout a porous sample without the need of harming electrically billed constructions inside the tissue. This enables two-four working day clearing of intact organs,
Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, megatomi.com rendering them thoroughly clear. This permits light penetration into your sample and makes certain the acquisition of large-resolution, in-concentrate image facts.
Megatome can be a novel microtome that permits for top-precision sectioning of an array of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with small tissue harm and knowledge reduction.
It’s time for you to up grade your microtome to Megatome. With correct superior-frequency slicing for an unmatched range of sample dimensions and types – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for varied programs.
The SmartSPIM light-weight sheet microscope gives unparalleled resolution and acquisition velocity during intact tissue volumes, though its modular layout allows you to up grade and customize specific components.
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SHIELD avoids the variability of hydrogel embedding and the knowledge decline from PFA preservation, protecting specimens for several rounds of processing.
Leverage the Crystal clear+ tissue clearing process, along with eFLASH and patented stochastic electrotransport technologies, to speedily crystal clear and label full organs. Important highlights and functions incorporate:
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